Total RNA sequencing is a technique used to determine the sequences of all RNA molecules within a cell or tissue, including messenger RNA (mRNA), transfer RNA (tRNA), ribosomal RNA (rRNA), and non-coding RNAs. This comprehensive approach provides a snapshot of the transcriptome, revealing the expression levels of genes, including which genes are turned on or off, and the abundance of different RNA species at a specific point in time. Total RNA sequencing is a powerful tool for investigating variations in gene expression across different cell types, tissues, or conditions within the same organism, offering insights into cellular processes, disease states, and responses to stimuli.
