RNA Sequencing

Charts the complete set of RNA molecules in a cell, offering insights into gene activity and the processes they control.
Total RNA-Sequencing
Total RNA sequencing is a technique used to determine the sequences of all RNA molecules within a cell or tissue, including messenger RNA (mRNA), transfer RNA (tRNA), ribosomal RNA (rRNA), and non-coding RNAs. This comprehensive approach provides a snapshot of the transcriptome, revealing the expression levels of genes, including which genes are turned on or off, and the abundance of different RNA species at a specific point in time. Total RNA sequencing is a powerful tool for investigating variations in gene expression across different cell types, tissues, or conditions within the same organism, offering insights into cellular processes, disease states, and responses to stimuli.
Total RNA-Sequencing
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mRNA Sequencing
It is a powerful technique used to determine the transcriptome of a cell or tissue by sequencing all the messenger RNA (mRNA) molecules. Since mRNA carries the coding information for proteins, mRNA sequencing reveals the expression levels of genes, indicating which genes are actively transcribed and how much mRNA is produced. This helps researchers understand gene activity, identify differentially expressed genes across different conditions or cell types, and study processes like gene regulation, alternative splicing, and the impact of various treatments or diseases.
mRNA Sequencing
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Small RNA Sequencing
Small RNA sequencing focuses specifically on sequencing small RNA molecules, typically less than 200 nucleotides in length. These small RNAs include various functional molecules like microRNAs (miRNAs), small interfering RNAs (siRNAs), and transfer RNAs (tRNAs), which play crucial roles in gene regulation, RNA interference, and other cellular processes. Small RNA sequencing allows researchers to identify and quantify these small RNAs, study their biogenesis and function, and investigate their involvement in diverse biological pathways and diseases, including cancer and developmental disorders.
Small RNA Sequencing
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Spatial Transcriptomics
Spatial transcriptomics, a cutting-edge technique within the realm of RNA sequencing, revolutionizes our understanding of gene expression by adding a crucial layer of information: location. Unlike traditional RNA sequencing, which analyzes gene expression in bulk or from isolated cells, spatial transcriptomics preserves the spatial context of RNA transcripts within intact tissues. This allows researchers to visualize how gene activity varies across different regions of a tissue, revealing intricate patterns and interactions that would otherwise be missed.
Spatial Transcriptomics
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Single-Cell RNA Sequencing (scRNA-seq)
Single-cell RNA sequencing (scRNA-seq) is a cutting-edge next-generation sequencing (NGS) technology that profiles the transcriptome of individual cells. Unlike bulk RNA-seq, which averages gene expression across many cells, scRNA-seq preserves cell-to-cell variation, enabling researchers to uncover rare cell types, developmental trajectories, and complex cellular behaviors.
Single-Cell RNA Sequencing
(scRNA-seq)
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