16S rRNA

Microbial
sequencing

What is 16S rRNA Sequencing?

16S ribosomal RNA (16S rRNA) is the RNA component of the 30S subunit of prokaryotic ribosomes. The 16S rRNA gene is highly conserved across bacteria but contains hypervariable regions that allow for species-level discrimination. Therefore, 16S rRNA sequencing is widely used to identify and classify bacteria and characterize microbial communities in environmental and clinical samples.

Organisms

Bacteria

Methodology

Sample Guideline

Sample Type	Concentration	Recommended Amount	Minimum Amount	Minimum volume (µl)	Storage (°C)	Buffer	Recommended RIN/DIN	Shipment	Storage tube
Soil/sediments	NA	500 mg	100 mg	NA	4	NA	NA	Ice pack
Liquid samples	NA	25-50 ml	NA	10,000	4	NA	NA	Ice pack
Feces (Human)	NA	Follow Kit instructions	Follow Kit instructions	NA	4/RT	NA	NA	Ice pack	Shared in collection kit tubes
Feces (Other species)	NA	100 mg	15-30 mg	NA	4/-20	NA	NA	Ice pack	Shared in collection kit tubes/1.5 ml tubes/ Cryovials
DNA	≥ 10 ng/µl	≥ 500 ng	150 ng	10-20	4/-20/-80	EB, or low TE (<0.1 mM EDTA)	>5	Ice pack	1.5 ml or 0.5 ml tubes
PCR purified products	≥ 20 ng/µl	≥ 1.5 µg		10-20	4/-20/-80	EB, or low TE (<0.1 mM EDTA)	NA	Ice pack	1.5 ml or 0.5 ml tubes

Analysis

16S rRNA

What is 16S rRNA Sequencing?

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