Small RNA sequencing focuses specifically on sequencing small RNA molecules, typically less than 200 nucleotides in length. These small RNAs include various functional molecules like microRNAs (miRNAs), small interfering RNAs (siRNAs), and transfer RNAs (tRNAs), which play crucial roles in gene regulation, RNA interference, and other cellular processes. Small RNA sequencing allows researchers to identify and quantify these small RNAs, study their biogenesis and function, and investigate their involvement in diverse biological pathways and diseases, including cancer and developmental disorders.
Human, Mouse, Plants, Fungi and other organisms
NGS
Sample Type | Concentration | Recommended Amount | Minimum Amount | Minimum volume (µl) | Storage (°C) | Buffer | Recommended RIN/DIN | Shipment | Storage tube |
---|---|---|---|---|---|---|---|---|---|
Tissue (Frozen) | NA | 100 mg | 15-30 mg | NA | -80 | RNA later/fresh/flash frozen | >7 | Dry ice | Preferably Cryovials |
Cell Pellet (Eukaryotic) | NA | 2-3 million cells | 1 million | NA | -80 | RNA later/fresh/flash frozen | NA | Dry ice | Preferably Cryovials |
Cell Pellet (Microorganisms) | NA | 100 million cells | 10 million (Depends on the organism and its size) | NA | -80 | RNA later/fresh/flash frozen | NA | Dry ice | Preferably Cryovials |
Blood | NA | 2500 µl | NA | 2500 | -80 | NA | >7 | Dry ice | PAXgene Tubes |
FFPE | NA | Block with enough material | NA | NA | 4 | NA | NA | RT or ice pack | NA |
RNA | ≥ 50 ng/µl | ≥ 1000 ng | 500 ng | 10-20 | -80 | NFW | >7 | Dry ice | 1.5 ml or 0.5 ml tubes |
cDNA | ≥ 10 ng/µl | ≥ 200 ng | 50 ng | 10-20 | 4/-20/-80 | EB, or low TE (<0.1 mM EDTA) | >5 | Ice pack | 1.5 ml or 0.5 ml tubes |