It is a powerful technique used to determine the transcriptome of a cell or tissue by sequencing all the messenger RNA (mRNA) molecules. Since mRNA carries the coding information for proteins, mRNA sequencing reveals the expression levels of genes, indicating which genes are actively transcribed and how much mRNA is produced. This helps researchers understand gene activity, identify differentially expressed genes across different conditions or cell types, and study processes like gene regulation, alternative splicing, and the impact of various treatments or diseases.
