ChipSeq is a combination of the chromatin immunoprecipitation technique with massively parallel sequencing. Chromatin immunoprecipitation allows the identification of specific DNA sequences that are bound to proteins of interest in vivo. ChIPSeq can be used to map DNA-binding proteins and histone modifications in a genome-wide manner at single-base-pair resolution
Human, Mouse, Plants, Bacteria, Fungi and other organisms
NGS
Sample Type | Concentration | Recommended Amount | Minimum Amount | Minimum volume (µl) | Storage (°C) | Buffer | Recommended RIN/DIN | Shipment | Storage tube |
---|---|---|---|---|---|---|---|---|---|
ChIP DNA | ≥ 10 ng/µl | 100 ng - 500 ng | 100 ng | 10-20 | 4/-20/-80 | EB, or low TE (<0.1 mM EDTA) | Ice pack | 1.5 ml or 0.5 ml tubes |