Whole Genome Sequencing

DNA
sequencing

What is Whole Genome Sequencing?

Whole Genome Sequencing (WGS) provides a comprehensive analysis of an organism’s entire genetic blueprint, encompassing both coding and non-coding regions. It facilitates the identification of genetic variants aiding in research and clinical applications. It also finds use in personalized medicine as it offers detailed genetic insights that can guide tailored treatments

Organisms

Human, Mouse, Plants, Bacteria, Fungi, and other organisms

Methodology

Sample Guideline

Sample Type	Concentration	Recommended Amount	Minimum Amount	Minimum volume (µl)	Storage (°C)	Buffer	Recommended RIN/DIN	Shipment	Storage tube
Tissue (Frozen)	NA	100 mg	15-30 mg	NA	-80	Fresh Frozen/Flash Frozen	NA	Dry ice	Preferably Cryovials
Cell Pellet (Eukaryotic)	NA	2-3 million cells	1 million	NA	-80	Fresh Frozen/Flash Frozen	NA	Dry ice	Preferably Cryovials
Cell Pellet (Microorganisms)	NA	100 million cells	10 million (Depends on the organism and its size)	NA	-80	Fresh Frozen/Flash Frozen	NA	Dry ice	Preferably Cryovials
Whole Blood	NA	500 µl	NA	200	4	NA	NA	Ice Pack	EDTA tubes/aliquoted in 1.5 ml tubes
Intact Genomic DNA	≥ 10 ng/µl	≥ 500 ng	150 ng	10-20	4/-20/-80	EB*, or low TE (<0.1 mM EDTA)	>5	Ice pack	1.5 ml or PCR tubes

Analysis

Whole Genome Sequencing

What is Whole Genome Sequencing?

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